Features and Benefits

Compatible with high-throughput handling systems.

General description

Neprilysin, also known as neutral endopeptidase, enkephalinase, CD10, and common acute lymphoblastic leukemia antigen, is a zinc-containing transmembrane metalloproteinase. It is able to hydrolyze very important endogenous peptides, such as natriuretic atrial factor, enkephalins, substance P, bradykinin and amyloid β (A β) peptide. Thus, NEP is a potentially therapeutic target in important pathological conditions such as cardiovascular disease, prostate cancer, and Alzheimer’;s disease. NEP has also been used as a biological marker in a type of childhood leukemia. The detection of NEP in endometrial stromal cells has been proposed as a helpful tool in the diagnosis of endometriosis. NEP is currently a focus of major interest in cardiovascular and neurological research.

Principle

The Neprilysin Activity Assay Kit utilizes the ability of an active NEP to cleave a synthetic substrate (Abz-based peptide) to release a free fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. The substrate is specific to NEP and can differentiate the NEP activity from Trypsin and other structurally similar zinc metalloproteinase in biological samples such as Angiotensin-Converting Enzymes (ACE1, ACE2), Endothelin Converting Enzymes (ECE1, ECE2). The assay kit is simple, specific and can detect as low as 20 µU/mg of NEP activity.

Suitability

Suitable for the measurement of Neprilysin activity in tissue homogenates (lung, kidney, etc.), cell culture (adherent or suspension cells)  and purified enzyme.